In 2019, the coronavirus pandemic was caused by severe acute respiratory syndrome coronavirus 2. In Taiwan, an inflatable negative pressure isolation chamber (INPIC) was applied into the emergency air medical transport (EAMT) in 2020. The standard operating procedure of the INPIC as infection control was developed and practiced in the UH 60M helicopter. There were five cases moved by the EAMT from the Lanyu Township and Ludao Township to Taiwan’s mainland. After they were screened positive for coronavirus disease 2019 (COVID 19) with a rapid test, the medical staff completed the INPIC preparation and loaded the patients into it before the EAMT. This study reported that none of the crewmembers were infected with COVID 19 during the transfers. However, some restrictions of the INPIC could be improved in the future, e.g. limited space, thermal stress, equipment immobilization, and communication barrier. The medical team should assess the efficiency and safety using the INPIC based on each patient’s condition before the flight.