Background: To evaluate the consistency of the thicknesses of corneal flaps created with the Amadeus and Moria M2 microkeratomes during laser in situ keratomileusis (LASIK). Methods: In this prospective study, 60 eyes underwent LASIK with the Moria M2 microkeratome and 100 eyes were cut using the Amadeus microkeratome. Keratometry readings and refractive errors were measured preoperatively. Corneal thickness was measured with an ultrasonic pachymeter before and during the flap procedure, and the difference was taken to be the flap thickness. Eyes were grouped for statistical analysis according to the order of blade use. The data were analyzed using a two-tailed t test and the Pearson correlation coefficient. Results: The mean central thickness of the corneal flap was 138.68±20.637μm in the Moria M2 130 group and 127.29± 20.873μm in the Amadeus group. There was a negative linear relationship between corneal flap thickness and the preoperative keratometric value in the Moria M2 130 group (r = -0.383, P = 0.002). There was a direct linear relationship between corneal flap thickness and preoperative corneal pachymetry in the Amadeus 140 group (r = 0.287, P = 0.004). Flap thickness was significantly thicker in the first eyes cut. Conclusions: Both the Moria M2 130 and the Amadeus microkeratomes produce accurate and repeatable corneal flaps. The Amadeus microkeratome achieved a statistically significantly lower deviation in flap thickness than that of the Moria M2 microkeratome.