儘管有許多人嘗試以生物防治法來防除雜草，但成功的例子不多。一種植物病原菌被研究人員選擇充作真菌殺草劑的候選對象之前，它首先必需通過室內與田間之「發展期」與「推展期」兩階段的嚴格篩選與測試。一般言知，施用真菌殺草劑的兩種主要策略是：(1)傳統使用法：即單純的引介一種真菌至特定雜草區域後，無需再重複的施用，真菌即可在雜草群以扼止雜草的繁殖；(2)鉅量使用法：每年需重複施用大的真菌至特定雜草區域，以殺死感病性的雜草。真菌殺草劑除具有較化學殺草劑窄的殺草範圍外，亦僅被應用在較難防除且親緣關係與農作物相近的雜草。目前，有兩種真菌殺草劑：Collego及DeVine已正式在美國商品化。

Although many attempts have been made for biological controlof weeds with plant pathogen , only a few cases have been successful.To develop mycoherbicides , candidate plant pathogens needbe severely tested through the developmental and deploymentalstages. Utilization of weed biocontrol agents is by means of c1assicaland inundative tactics. Mycoherbicides have a narrower controlspectrum than chemical herbicides , and would mainly be usedon hard-to-control weeds that are botanically c10sely related tothe crop. Two mycohericides , Collego and De Vine , have beencommercialized in United States.