本研究利用百慕達草Tifway-419草坪爲材料，探討不同氮肥用量及刈割頻度對其色素含量及遙測光譜的影響。百慕達草Tif-way 419之葉綠素生合成相關色素，包括Chl a+b、total porphyrin、Chlide a+b、Phe a+b、含植醇及脫植醇色素含量，以及類胡蘿蔔素含量皆隨著氮肥用量增加而增多；在個別吡啉莫耳百分比的變化趨勢方面，受到氮肥用量的影響不明顯。百慕達草Tifway 419之植被反射光譜，在400-700nm間之反射比都很低，最大反射比發生在近紅外光約750nm以上之波段。近紅外光反射比隨著氮肥用量的增加而升高，而可見光區域之反射比則呈相反趨勢，隨著氮肥用量增加而下降。NDVI680、NDVI705及模擬衛星寬波段之NDVIbroad與三種色素Chl a、Chl b及Car含量間具有高相關性，其R^2皆爲0.7左右(p<0.01)。除Chl b外，含有植醇鏈(phytol chain)的色素比無植醇者對NDVI之貢獻較大，即LP Car較MP Car對NDVI之貢獻較大。資料分析進一步顯示，葉綠素與類胡蘿蔔素之生合成與崩解代謝物中，位於葉綠體類囊膜(thylakoid membrane)上極性較小的色素對植被反射光譜NDVI之貢獻性較大；而分佈於基質(stroma)之水溶性較大之色素，其對NDVI之貢獻較小。

The objectives of this study were conducted to examine the contents of pigments in the leaves and vegetation reflectance spectrum of bermudagrass "Tifway 419" (Cynodon transvaalensis x C. dactylon) with different nitrogen application rates and mowing frequencies. The contents of chlorophyll (Chl), its biosynthetic and degradative intermediates (total porphyrin; chlorophyllide, Chlide; pheophytin, Phe; phytylated and dephytylated pigments), and carotenoids in the leaves of bermudagrass Tifway 419 elevated with increasing nitrogen rates. The mole percents of three porphyrins (protoporphyrin, PPIX; magnesium protoporphyrin IX, MGPP; protochlorophyllide, Pchlide) were not influenced by nitrogen application rate significantly. The canopy reflectance spectra of bermudagrass Tifway 419 were measured. The reflectance in the range of 400 to 700 nm was very low, while near-infrared (NIR) band above 750 nm was very high. The reflectance in the near-infrared wavebands was increased with increasing nitrogen application rate, but the reflectance in the visible region was decreased as nitrogen application rate increased. Spectral indices NDVI680, NDVI705, and NDVIbroad calculated using broad-band reflectance were correlated well with the contents of Chl a, Chl b, and Car (R^2 about 0.7, p<0.01). The chlorophyll-related compounds containing phytol chain greatly contributed to changes in NDVI than those compounds without phytol chain, except for Chl b. The LP Car also played a more important role in NDVI change than the MP Car. Results suggest that NDVI calculated from vegetation reflectance spectra was more affected by the chlorophyll- and carotenoid-related compounds located onto the thylakoid membranes than those located in the stroma of chloroplast.